A Robust, High-Throughput Protocol for Deriving Microglia From Human Stem Cells
NYSCF Research Institute scientists have developed a robust, efficient method for deriving microglia, the immune cells of the brain, from human stem cells. Microglia are increasingly implicated in neurological disorders including Alzheimer’s disease, Parkinson’s disease and multiple sclerosis, among many others. However, research into the role of human microglia in these disorders has long been hampered by the inability to obtain them from the human nervous system.
Published in Stem Cell Reports, this new protocol now enables scientists around the world to generate this critical cell type from individual patients and improve our understanding of the role of microglia neurological malfunction.
NYSCF scientist Dr. Panos Douvaras was first author on the paper and NYSCF scientists Dr. Scott Noggle and Dr. Valentina Fossati were co-senior authors. The protocol was developed in collaboration with colleagues from the NYSCF Research Institute and others at the Icahn School of Medicine at Mount Sinai with support from National Institute on Aging (NIA).